OptoElectronic LabChip for Cancer
Immunotherapy
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Introduction The first line of
defense for maintaining a healthy human body is the immune system, whose
necessary responses of single cell and intercellular interactions function
through versatile mechanisms. However, effector natural killer (NK) cells in
the immune system could be in direct contact with target cells and cause
their death. Immune cells execute their physical activities through versatile
mechanisms where cell–cell interaction is one of the important
mechanisms. Furthermore, through observing the immune cytotoxic activity of
individual NK cell during the interaction with the target cell, the overall
performance of immune system could be predicted. Manipulating a single cell
in a suitable environment can provide an immediate and direct observation of
its cellular behavior. Recently, using microfluidic techniques to manipulate
and observe single cells has been widely applied to clinical research, such
as drug and toxicity screening,6 tissue engineering, and cancer
immunotherapy. NK cells are effector cells accounting for approximately 5–15%
of peripheral blood lymphocytes and they play important roles in the innate
immune system inside the human body. A cancer
immunotherapy μ-environment LabChip, equipped with titanium oxide
phthalocyanine (TiOPc)-based optoelectronic tweezers (OET) to achieve direct
cell–cell contact, is developed to study the interaction between immune
cells and other cells for real-time analysis of NK cells' behavior. The
illustration for cancer immunotherapy μ-environment
LabChip, taking advantage of optoelectronic tweezers, developed in our group is
shown in the following, which is a cover image from our published paper
accepted by Lab on Chip. |
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Selected Publication |
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[1] Ling-Yi Ke,
Zong-Keng Kuo, Yu-Shih Chen, Tsu-Yi Yeh, Minxiang Dong, Hsiang-Wen Tseng and
Cheng-Hsien Liu, “Cancer
Immunotherapy m-environment LabChip:
Taking Advantage of Optoelectronic Tweezers,” Lab on a Chip, 2018, 18, 106-114 (January
2018, Issue 1) |