Liver on LabChip

A laser diffraction-induced dielectrophoresis (DEP) phenomenon for the patterning and manipulation of individual HepG2 cells and polystyrene beads via positive/negative DEP forces is developed. The optoelectronic substrate was fabricated by organic photoconductive material, TiOPc, via the spin-coating process on the indium tin oxide glass surface. A piece of square aperture array grid grating was utilized to transform the collimating He-Ne laser beam into the multi-spot diffraction pattern which forms the virtual electrodes as the TiOPc-caoting surface was illuminated by the multi-spot diffraction light pattern. HepG2 cells were trapped at the spot centers and polystyrene beads were trapped within the dim region of the illuminating image. The Fresnel diffraction image illustrated the distribution of trapped microparticles. This concept of utilizing laser diffraction image to generate virtual electrodes on our TiOPc-based optoelectronic DEP chip extends the applications of optoelectronic dielectrophoretic manipulation.


Demonstration (by Dr. Chen-Ta Ho at Micro-Systems and Control Lab.)


*   Liver-cell patterning Lab Chip: mimicking the morphology of liver lobule tissue



Selected Publication

[1] Chen-Ta Ho, Ruei-Zeng Lin, Rong-Jhe Chen, Chung-Kuang Chin, Song-En Gong, Hwan-You Chang, Hwei-Ling Peng, Long Hsu, Tri-Rung Yew, Shau-Feng Chang and Cheng-Hsien Liu, “Liver-cell patterning Lab Chip: mimicking the morphology of liver lobule tissue,” Lab on Chip, 2013, 13, 3578-3587 (21 September 2013, Issue 18)

[2] Chen-Ta Ho , Ruei-Zeng Lin, Wen-Yu Chang, Hwan-You Chang , Cheng-Hsien Liu, “Rapid heterogeneous liver-cell on-chip patterning via the enhanced field-induced dielectrophoresis trap,” Lab on a Chip, 2006, 6, 724 – 734