Lung on LabChip
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Introduction We develop a variety
of biomimetic microsystems that reconstitute the lung microenvironment for the
studies of lung inflammation, chronic obstructive asthma (COA), Asthma acute
exacerbation, lung cancer target therapy. For lung inflammation, we monitoring
the role of eosinophil cationic protein (ECP), which induces the airway
epithelial cell expression of CXCL-12. That in turn stimulates the migration
of fibrocytes towards the epithelium.
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For chronic
obstructive asthma (COA) and during asthma acute exacerbation, we
investigated whether these chemoattractants and S100A9, through the receptor
for advanced glycation endproducts are involved in fibrocyte trafficking in
patients with chronic obstructive asthma (COA) and during an acute
exacerbation (AE) in patients without airflow obstruction (Asthma AE group). We
collected peripheral blood from the asthmatic patients with normal pulmonary
function, the patients with COA, the patients in the Asthma AE group, and the
healthy subjects. Isolated circulating fibrocytes were used for migration assay.
We used our developed lung chip to study the role of the CXCL12/CXCR4 and
CCL19/CCR7 axes in fibrocyte migration. |
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Transmigration of
fibrocytes were detected via our developed lung chip by using microscopy as
shown in A. Transmigration of fibrocytes from patients in the Asthma AE group
in the presence of CXCL12, anti-CXCR4 antibodies, and anti-CCR7 antibodies
are shown in B. Transmigration of CFSE-labeled fibrocytes from patients in the
Asthma NPF or COA groups are shown in C. Transmigration of fibrocytes from
patients with COA in response to CCL19, anti-CCR7 antibodies, and anti-CXCR4
antibodies are show in D.
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The illustration for
lung on a Labchip is shown in the following, which is a cover image from our
published paper accepted by Integrative
Biology.
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Selected Publication |
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[1] Tushar H.
Punde, Wen-Hao Wu, Pei-Chun Lien, Ya-Ling Chang, Ping-Hsueh Kuo, Margaret
Dah-Tsyr Chang, Kang-Yun Lee, Chien-Da Huang, Han-Pin Kuo, Yao-Fei Chan,
Po-Chen Shih, Cheng-Hsien Liu,” Biologically inspired lung-on-a-chip
for studying protein-induced lung inflammation”, Integrative Biology, 2015 Feb., 7, 162-169 [2] Chun-Hua
Wang, Tushar Harishchandra Pund, Chien-Da Huang, Pai-Chien Chou, Tzu-Ting
Huang, Wen-Hao Wu, Cheng-Hsien Liu, Kian Fan Chung and Han-Pin Kuo,
“Fibrocyte trafficking in patients with chronic obstructive asthma and
during an acute asthma exacerbation,” “Fibrocyte trafficking in
patients with chronic obstructive asthma and during an acute asthma
exacerbation,” Journal of Allergy
and Clinical Immunology, May 2015 Volume 135, Issue 5, Pages
1154–1162.e5 |