Optoelectronic Manipulation and Control


We develop light-driven optoelectronic tweezers based on the organic photoconductive material titanium oxide phthalocyanine. These tweezers function based on either positive or negative dielectrophoresis (nDEP). The dynamic manipulation of a single microparticle and cell patterning are demonstrated by using this light-driven optoelectronic DEP chip. The adaptive light patterns that drive the optoelectronic DEP onchip are designed by using Flash software to approach appropriate dynamic manipulation. This is also the first reported demonstration, to the best of our knowledge, for successfully patterning such delicate cells from human hepatocellular liver carcinoma cell line HepG2 by using any optoelectronic tweezers. 

Ref: Dynamic manipulation and patterning of microparticles and cells by using TiOPc-based optoelectronic dielectrophoresis  [full paper]


A laser diffraction-induced dielectrophoresis (DEP) phenomenon for the patterning and manipulation of individual HepG2 cells and polystyrene beads via positive/negative DEP forces is developed. The optoelectronic substrate was fabricated by organic photoconductive material, TiOPc, via the spin-coating process on the indium tin oxide glass surface. A piece of square aperture array grid grating was utilized to transform the collimating He-Ne laser beam into the multi-spot diffraction pattern which forms the virtual electrodes as the TiOPc-caoting surface was illuminated by the multi-spot diffraction light pattern. HepG2 cells were trapped at the spot centers and polystyrene beads were trapped within the dim region of the illuminating image. The Fresnel diffraction image illustrated the distribution of trapped microparticles. This concept of utilizing laser diffraction image to generate virtual electrodes on our TiOPc-based optoelectronic DEP chip extends the applications of optoelectronic dielectrophoretic manipulation.





Demonstration (by Dr. Shih-Mo Yang- collaborated with Prof. Long Hsu’s Group)

*   Magnetic Microparticles Concentration-MSCL Lab (Optoelectronic Tweezers, OET)

*   Microparticles Collection-MSCL Lab (Optoelectronic Tweezers, OET)

*   Liver Cells Patterning-MSCL Lab (Optoelectronic Tweezers, OET)

*   Conical Flask-MSCL Lab (Optoelectronic Tweezers, OET)

*   Word Transformation-MSCL Lab (Optoelectronic Tweezers, OET)

*   Microparticles Sweeper-MSCL Lab (Optoelectronic Tweezers, OET)

*   Spinning, Rotating and Dancing Collision of Liver Cells-MSCL Lab (Optoelectronic Tweezers, OET)

*   Sides Cells Squeeze Center Cell-MSCL Lab (Optoelectronic Tweezers, OET)

*   4x3 Liver Cells Array-MSCL Lab (Optoelectronic Tweezers, OET)

*   Microparticle Sorting with Light-Pattern-Based Switch-MSCL Lab (Optoelectronic Tweezers, OET)

*   Microparticles in Fan-MSCL Lab (Optoelectronic Tweezers, OET)

*   Gas Bubble Manipulation With Light Image-MSCL Lab (Optoelectronic Tweezers, OET)

Selected Publication

[1] Shih-Mo Yang, Sheng-Yang Tseng, Hung-Po Chen, Long Hsu, and Cheng-Hsien Liu, “Cell patterning via diffraction-induced optoelectronic dielectrophoresis force on an organic photoconductive chip,” Lab on Chip, 2013,13, 3893-3902 (07 October 2013, Issue 19)

[2] Tung-Ming Yu, Shih-Mo Yang, Chien-Yu Fu, Ming-Huei Liu, Long Hsu, Hwan-You Chang, Cheng-Hsien Liu, “Integration of organic opto-electrowetting and poly(ethylene) glycol diacrylate(PEGDA) microfluidics for droplets manipulation,” Sensors and Actuators B: Chemical Volume 180, April 2013, Pages 35–42.

[3] Shih-Mo Yang, Tung-Ming Yu, Hang-Ping Huang, Meng-Yen Ku, Sheng-Yang Tseng, Che-Liang Tsai, Hung-Po Chen, Long Hsu and Cheng-Hsien Liu, “Light-driven manipulation of pico-bubbles via optoelectronic dielectrophoresis,” Applied Physics Letters, Volume 98, Issue 15, 153512 (2011); doi:10.1063/1.3580760

[4] Shih-Mo Yang, Tung-Ming Yu, Hang-Ping Huang, Meng-Yen Ku, Long Hsu, and Cheng-Hsien Liu*, “Dynamic manipulation and patterning of microparticles and cells by using TiOPc-based optoelectronic dielectrophoresis,” Optics Letters, Vol. 35, No. 12, 1959, 2010