Optoelectronic Manipulation and Control
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Introduction We develop
light-driven optoelectronic tweezers based on the organic photoconductive
material titanium oxide phthalocyanine. These
tweezers function based on either positive or negative dielectrophoresis
(nDEP). The dynamic manipulation of a single microparticle and cell patterning are demonstrated by
using this light-driven optoelectronic DEP chip. The adaptive light patterns
that drive the optoelectronic DEP onchip are
designed by using Flash software to approach appropriate dynamic
manipulation. This is also the first reported demonstration, to the best of
our knowledge, for successfully patterning such delicate cells from human
hepatocellular liver carcinoma cell line HepG2 by using any optoelectronic
tweezers. Ref: Dynamic manipulation and patterning of microparticles
and cells by using TiOPc-based optoelectronic dielectrophoresis [full paper]
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A laser diffraction-induced dielectrophoresis
(DEP) phenomenon for the patterning and manipulation of individual HepG2
cells and polystyrene beads via positive/negative DEP forces is developed.
The optoelectronic substrate was fabricated by organic photoconductive
material, TiOPc, via the spin-coating process on
the indium tin oxide glass surface. A piece of square aperture array grid
grating was utilized to transform the collimating He-Ne laser beam into the
multi-spot diffraction pattern which forms the virtual electrodes as the TiOPc-caoting surface was illuminated by the multi-spot
diffraction light pattern. HepG2 cells were trapped at the spot centers and
polystyrene beads were trapped within the dim region of the illuminating
image. The Fresnel diffraction image illustrated the distribution of trapped microparticles. This concept of utilizing laser
diffraction image to generate virtual electrodes on our TiOPc-based
optoelectronic DEP chip extends the applications of optoelectronic dielectrophoretic manipulation.
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Selected Publication |
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[1] Shih-Mo
Yang, Sheng-Yang Tseng, Hung-Po Chen, Long Hsu, and Cheng-Hsien Liu,
“Cell patterning via diffraction-induced optoelectronic dielectrophoresis force on an organic photoconductive
chip,” Lab on Chip, 2013,13,
3893-3902 (07 October 2013, Issue 19) [2] Tung-Ming
Yu, Shih-Mo Yang, Chien-Yu Fu, Ming-Huei Liu, Long Hsu, Hwan-You Chang, Cheng-Hsien Liu,
“Integration of organic opto-electrowetting
and poly(ethylene) glycol diacrylate(PEGDA)
microfluidics for droplets manipulation,” Sensors and Actuators B: Chemical Volume 180, April 2013, Pages
35–42. [3] Shih-Mo
Yang, Tung-Ming Yu, Hang-Ping Huang, Meng-Yen Ku,
Sheng-Yang Tseng, Che-Liang Tsai, Hung-Po Chen,
Long Hsu and Cheng-Hsien Liu, “Light-driven manipulation of
pico-bubbles via optoelectronic dielectrophoresis,”
Applied Physics Letters, Volume 98,
Issue 15, 153512 (2011); doi:10.1063/1.3580760 [4] Shih-Mo
Yang, Tung-Ming Yu, Hang-Ping Huang, Meng-Yen Ku,
Long Hsu, and Cheng-Hsien Liu*, “Dynamic manipulation and patterning of
microparticles and cells by using TiOPc-based optoelectronic dielectrophoresis,”
Optics Letters, Vol. 35, No. 12,
1959, 2010 |